Published on: May 13, 2025

Several data present in literature have already demonstrated some beneficial effects of nucleotides in enhancing quality of life and general health. As the global population ages, maintaining cognitive sharpness, muscle strength, and metabolic health becomes increasingly vital.  Recently, Prosol commissioned a clinical study to verify nucleotides ability to improve distinct physiological domains in aging population.
The aim of this new study, published on Nutrients, was to examine the effects of the administration of dietary nucleotides—natural compounds found in yeast—in improving neurogenesis, physical performance, inflammatory modulation and body composition in older adults.

The data obtained made it possible to highlight the important roles nucleotides play in human metabolism.

The 10-week trial involved 69 physically independent adults aged 60–75 who maintained spontaneous physical activity (e.g., walking, household chores).

Participants were divided into three groups:

1. Yeast Nucleotide (YN) Group received RiboDIET^® (250 mg/day)
2. Neuro-Based Nucleotide (NF) Group received RiboCARE^® Neuro (150 mg/day)
3. Placebo Group

 

Figure 1. The table provides a concise summary of the main expected effects of this supplementation in older adults with an active lifestyle, based on percentage changes in each variable according to statistical significance and effect size. For a more comprehensive statistical interpretation of this figure, the reader is referred to the tables provided in the Results section. YN: RiboDIET® solution. NF: RiboCARE® Neuro solution.

 

Cognitive Function Enhancement

RiboCARE^® Neuro was associated with an 18% improvement in executive function, as measured by the Trail Making Test B. This enhancement correlated with increased levels of brain-derived neurotrophic factor (BDNF), a neurotrophin critical for neuroplasticity and synaptic function.
Both RiboCARE^® Neuro and RiboDIET^® outperformed placebo in measures of processing speed and cognitive flexibility, indicating a broader cognitive benefit.

• RiboCARE^® Neuro appears particularly suitable for individuals seeking targeted cognitive enhancement.
• RiboDIET^® provides complementary support through a broader nutrient matrix.

Anti-Inflammatory and Antioxidant Benefits

Participants supplemented with RiboDIET^® exhibited significant reductions in key inflammatory cytokines:

• Interleukin-6 (IL-6): −9.8%
• Tumor necrosis factor-alpha (TNF-α): −13.9%

In both supplement groups, oxidative stress markers (F2-isoprostanes) decreased by 37%, while intracellular levels of glutathione — a major endogenous antioxidant — increased by 17%.

• RiboDIET^® is indicated for inflammatory conditions and systemic immune support.
• RiboCARE^® Neuro contributes to the mitigation of oxidative damage and supports redox balance.

Improved Physical Performance

Mobility and muscular function were also positively influenced. In the Timed Up and Go Test, RiboCARE^® Neuro users showed a 10.3% improvement, compared to 5.9% with RiboDIET^®.
Moreover, both groups demonstrated a 30% increase in lower-body endurance (assessed via the 30-second Chair Stand Test) relative to placebo.

• RiboCARE^® Neuro may be particularly effective for supporting mobility and fall prevention.
• RiboDIET^® contributes to general physical capacity and endurance maintenance.

Body Composition and Metabolic Health

Unlike placebo participants — who experienced an increase in fat mass (+5%) and a decline in lean muscle mass (−2.2%) — individuals in the RiboCARE® Neuro and RiboDIET® groups maintained stable body composition throughout the intervention.

• RiboDIET^®, with its comprehensive nutrient profile, may aid metabolic regulation.
• RiboCARE^® Neuro supports energy metabolism and lean mass preservation at the cellular level.

 

These results underscore the potential of nucleotides as effective, science-backed ingredients for developing supplements aimed at supporting healthy aging: the study confirms that nucleotide supplementation—particularly when combined with light physical activity—can deliver measurable benefits on muscle function, cognitive health, metabolic balance, and inflammatory status in older adults.

Individually or in synergy, RiboCARE^® Neuro and RiboDIET^® offer tailored strategies for those seeking scientifically grounded nutritional support for aging well.
For companies formulating next-generation products for the senior population, Prosol’s nucleotide-based solutions offer a proven, natural approach to promoting vitality, mobility, and resilience against age-related decline. This research provides a strong foundation for innovation in the healthy aging supplement category.

Published on: Oct 25, 2021

In 2019 Prosol announced the promising result of a first study on the role of dietary nucleotides (Ribodiet^®) in Alzheimer disease. Last July a scientific study on the same topic was published on Biomedicine and Pharmacotherapy review; the study showed extremely interesting benefits of RIBODIET® in alleviating memory decline, neuroinflammation and oxidative stress.

Alzheimer’s disease is one of the most common form of dementia, characterized by the deposition of extracellular amyloid-β (Aβ) peptides in the brain. The neuronal death, that characterizes this neurodegenerative disorder, causes irreversible cognitive impairment and behavioral alteration.

The option of natural-derived products and nutraceuticals is scientifically solid and to be considered for a future medical option in industrialized countries. Natural products are not an alternative to drugs but, on the contrary, can be helpful to complement the pharmacological therapy and prevent the onset of chronic diseases.

In this context, has been showed that Ribonucleotides are able to improve the clinical outcomes in immunosuppressive and inflammatory-based diseases.

A recent study[1] evaluated the effects of a ribonucleotides-based ingredient (Ribodiet^®) in a non-genetic mouse model of AD. Given the strict involvement of nucleosides and nucleotides on neuronal and cognitive functions, the team of the study[2] decided to examine whether a ribonucleotides-based ingredient could ameliorate Aβ‐induced neuro-inflammation and oxidative stress.

The study presented the first and complete overview of how the supplementation with a ribonucleotide-based ingredient (Ribodiet^®) may have a positive impact on synaptic degeneration in Alzheimer Disease and in controlling the most common form of neurodegenerative disease. In particular the study showed that:

• Ribodiet^® lessens oxidative stress and brain inflammation
• Ribodiet^® modulates amyloid pathology in a murine model of Alzheimer disease
• Ribonucleotide-based ingredients are effective on neuro-inflammatory-based disease/s

These results lay the first pillar for a rationale use of ribonucleotide-based ingredients for the treatment of Alzheimer disease and other age-related diseases. The study was published on Biomedicine & Pharmacotherapy.  You can view and download full text PDF.

To learn more about Ribodiet please click here or contact Prosol Spa:
Via Carso, 99 – 24040 MADONE (BG), Italy.
Tel +39 035.99.16.65
E-mail: prosol@prosol.it

[1] Original article – Supplementation with ribonucleotide-based ingredient (Ribodiet®) lessens oxidative stress, brain inflammation, and amyloid pathology in a murine model of Alzheimer on Biomedicine & Pharmacotherapy, Volume 139, July 2021, 111579

[2] Anella Saviano, GianMarco Casillo, Federica Rauccia, Alessia Pernice, Cristina Santarcangelo, Marialuisa Piccolo, MariaGrazia Ferraro, Miriam Ciccone, Alessandro Sgherbini, Nadia Pedretti, Daniele Bonvicini, Carlo Irace, Maria Daglia, Nicola Mascolo, Francesco Maione.

Numerous data present in literature have already confirmed some positive effects of nucleotides in reducing inflammation. Recently, Prosol commissioned a new in-vitro study to verify nucleotides ability to improve muscle cell function. The intent of this new study[1]  published on the Journal of Food Science and Nutrition Research, was to observe the effects of a blend of pure nucleotides (Ribocare®) and an yeast extract rich in nucleotides (Ribodiet®) on energy metabolism and contractility.

In this study, the cellular mechanisms triggered on muscle cells by nucleotides used as food supplements were verified for the first time. The new test reports made it possible to discover and verify for the first time the important roles they played.

[1]  Francesca Uberti, Sara Ruga, Vera Morsanuto, Rebecca Galla, Mahitab Farghali, Claudio Molinari. Role of Ribonucleotides in Improving Muscle Cell Function. Journal of Food Science and Nutrition Research 3 (2020): 231-251.

Prosol ingredients Ribodiet® and Ribocare® MIX were submitted to the test.

Ribodiet® is a yeast extract standardized in free nucleotides (>40%) source also of macro and micronutrients naturally occurring in yeast cell (AminoAcids, minerals, group B vitamins). Ribocare® MIX is a blend of pure nucleotides 20% each (AMP+CMP+GMP+UMP+IMP)

All the mix were used without solvents and loose directly in the medium used to stimulate the cells at the exact concentration corresponding to human dosages present in baby milk.

In light of the significant results obtained, supplementary experiments investigated the effects of the same nucleotides under strict exercise conditions with the purpose to recognize the correct human use of nucleotides as food supplements to better sustain the exercise effects. The results obtained show for the first time, a direct effect of nucleotides on energy metabolism and calcium/ magnesium movements during in vitro exercise simulation and provide useful indications to support muscle cells during exercise or during aging.

The results are published on Journal of Food Science and Nutrition Research 3 (2020): 231-251.

The latest studies may advocate new nutritional solutions through food supplements able to sustain physical exercise and to limit fatigue and physical activity side effects, using nucleotides. Nucleotides are indeed a natural compound, characterized by a bigger compliance for muscle cells, as they are involved in numerous functions.

A brief test report of the study is prompt available over the Prosol website: fill in the form to download it.

More info?

Obtain a full copy of the trial study here

Several studies have already demonstrated that dietary nucleotides can have beneficial effects on gut health. In particular, has been shown that those can have a positive impact on gastric mucosa integrity and reparation,  on  improving intestinal immune defences and on promoting useful bacteria growth as well.

Recently, Prosol commissioned a new in-vitro study to verify Ribodiet®’s ability to boost intestinal wellness.

The aim of this study was to examine the effects of a mixture of nucleotides in modulating gut barrier functionality and integrity.

Ribodiet® is a yeast extract standardized in a high content of free nucleotides (>40%), rich in all macro and micro nutrients naturally occurring in a yeast cell: essential and non essential aminoacids, minerals and group B vitamins. Recently Ribodiet properties has been evaluated by a new in vitro model.

In the last study in particular, external scientists and experts[1]  examined Ribodiet® power in modulating gut barrier functionality and integrity. The study was conducted on a 3D Caco-2 cell line as a model of the intestinal barrier.

The study has confirmed that the somministration of a mixture of nucleotides has significant effects on modulating gut barrier functionality and integrity: those results strengthen what has already been described in the literature.

[1]Laboratory: EcamRicertLab, Padoa 2019 – Scientific Ref.: Dott. Benetti

The report is now available for free over the Prosol website: fill in the form to download the results. To learn more about the entire publication (in vitro and clinical studies, applications, dosages, etc …) and for supporting documentation contact us.

More info?

Obtain a full copy of the trial study here